Development of an Electrochemical Aptasensor for Detection of Ochratoxin A Okratoksin A Tayinine Yönelik Elektrokimyasal Aptasensör Geliştirilmesi
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چکیده
Çalışmada Okratoksin A’nın (OTA) işaretsiz bir yöntemle tayinine yönelik elektrokimyasal aptasensör tasarımı gerçekleştirilmiştir. 5’ ucundan tiyohekzil işaretli (SH-(CH 2 ) 6 ) aptamer dizileri tek kullanımlık perde baskılı altın elektrot (AuSCPE) yüzeylerine tiyol grubu ile bağlanmıştır. OTA ve DNA aptamer dizisi arasındaki bağlanma Elektrokimyasal Empedans Spektrometri tekniği (EIS) ile 5mM [Fe(CN) 6 ] çözeltisi varlığında elektron aktarımına dayalı olarak tayin edilmiş ve tayin sınırı da 0.375 nM olarak saptanmıştır.
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تاریخ انتشار 2017